亚洲中文无码,久久久久国產麻豆无碼AV,自拍偷自拍亚洲精品播放,亲爱的老师4韩剧免费观看

技術(shù)文章 / article 您的位置:網(wǎng)站首頁 > 技術(shù)文章 > TWISTDX RPA試劑盒((加MMLV和抑制劑)操作建議

產(chǎn)品列表

PROUCTS LIST

TWISTDX RPA試劑盒((加MMLV和抑制劑)操作建議

發(fā)布時間: 2020-05-12  點擊次數(shù): 2103次

英國TwistDx公司(前身為ASM科技有限公司)成立于1999年,基于英國劍橋Babraham研究院建立的生物技術(shù)公司。該公司通過突破等溫DNA擴(kuò)增,開發(fā)的重組酶聚合酶擴(kuò)增*技術(shù)(RPA),被譽為DNA診斷領(lǐng)域的革命性創(chuàng)新。

RT試劑盒舉例

(1)Revert Reverse Transcriptase(200U/uL)(Thermo EP0441)

(2)RNA Inhibitor (40,000 U/mL)( Enzymatics Y9240L

TWISTDX廠家回復(fù)

  “We recommend using similar RT amounts to that of a PCR reaction of the same volume, according to your chosen manufacturer’s instructions.

  We also suggest that you run reactions at 40°C and delay agitation of the reaction by a minute to give the RT time to work, otherwise you just run the reaction as you would a normal TwistAmp® reaction (it’s a one-step process).

  It’s also advisable to add RNase Inhibitor to any reaction where RNA is the target material. Any commercially available RNase Inhibitor will be suitable.

  If the M-MLV RT that you are using comes with a separate dilution/reaction buffer, we suggest not using it as it will lower the concentration of the enzyme.

  The “delay agitation of the reaction by a minute” refers to the first mixing step per the protocol in the manual (Step 4). So wait 5 minutes instead of 4 minutes before “vortex, spin down briefly, and return the samples to the reader ensuring that the tubes are returned to their original positions in the block.”

  If we were to try and use it again, we would do a titration to determine optimal quantity to add in the new formulation reactions (we haven’t used it since we changed the formulation of the reactions a couple of years ago).

  When using RNase Inhibitor. We have used 1µg /50µl reaction, some R&D users have used 2µg /50ul, which didn’t appear to have a negative effect on the performance in our own assay.

  For this : RNAse Inhibitor, that manufacture uses 10 ul of the RNAse solution in 50 ul reaction volumes for activity determination, but your customer can try different amounts 5ul or 20ul to see what will give the best results. For this product, references of 50 ul PCR reaction volumes would also be reasonable.

TwistDx以此技術(shù)為基礎(chǔ)生產(chǎn)的核酸擴(kuò)增類產(chǎn)品,能在恒定且較低的溫度下,僅用10~20分鐘,進(jìn)行單分子核酸檢測。這一技術(shù)對環(huán)境、硬件設(shè)施的要求很低,對于生物防護(hù)、水體檢驗、食品檢驗、醫(yī)療診斷、微流體、獸醫(yī)等方面都有良好的應(yīng)用。

RPA擴(kuò)增的各種體系試劑盒中,含有DNA、RNA擴(kuò)增所需的所有試劑,研究者只要準(zhǔn)備好引物和模板就行了。擴(kuò)增結(jié)果可以分別通過凝膠電泳檢測、熒光定量檢測或試紙條檢測,產(chǎn)物純化后可用于下游研究。

默瑞生物

默瑞(上海)生物科技有限公司,作為專業(yè)的生命科學(xué)產(chǎn)品推廣與服務(wù)商,以“創(chuàng)新,專業(yè),共贏”的理念,不斷強化服務(wù)體系,提升客戶體驗,以莫納生物產(chǎn)品為主線,結(jié)合國內(nèi)外優(yōu)質(zhì)產(chǎn)品提供高標(biāo)準(zhǔn)完整解決方案,幫助科研客戶和生物技術(shù)企業(yè)高效創(chuàng)新,加速轉(zhuǎn)化,創(chuàng)造更多優(yōu)質(zhì)產(chǎn)品,提升社會福祉,實現(xiàn)多方共贏。

  默瑞生物創(chuàng)立以來,已經(jīng)陸續(xù)導(dǎo)入TwistDx,enzymatics,KAPABIOSYSTEMS,BioDynami,Jackson,Echelon,ChromoTek,Nanocs,Biotechrabbit,LeeBiosolutions, magtivio等優(yōu)質(zhì)品牌,逐步培育成為專業(yè)用戶的選擇。

在線客服 聯(lián)系方式

服務(wù)熱線

17317196276

欧美三级乱人伦电影| 人妻无码久久中文字幕专区| 国产成人亚洲精品无码| 精品人妻一区二区三区四区| 无码久久久久久久久| 国产剧情av精品迷奷| 一色一伦一区二区三区| 亚洲综合AV一区二区三区 | 东京热无码免费a片免费下载 | 成人AV无码一区二区三区| 久久精品A亚洲国产V高清不卡| 无码国产69精品久久久久网站| JIZZ大全欧美| 亚洲欧美精品无码一区二区三区| 欧美成人看片一区二区三区尤物| 久久99精品久久久久久| 浴室人妻的情欲hd三级| 亚洲AV无码精品狠狠爱| 亚洲AV片不卡无码久久| 国产一区二区在线观看| 亚洲AV永久无码一区二区三区| 扒开腿狂躁女人视频免费| 国产+成+人+亚洲欧洲自| 亚洲 欧洲 日产| 无码亚洲精品无码专区| 色精阁久艾草国产WWW| 亚洲精品国产精品国自产| 免费web服务器网站| 久久久久亚洲av成人无码网站| 国产av旡码专区亚洲av苍井空| 亚洲精品久久无码| 久久精品久久久久久久精品| 漂亮人妻洗澡被公强| 好男人资源在线看免费的| 无码人妻精品一区二区三区久久久| 欧美不卡一区二区三区| 亚洲av永久无码精品| 少妇人妻一区二区三区视频 | 男男啪啪激烈高潮cc漫画免费| 日韩亚洲AV人人夜夜澡人人爽| 99无码熟妇丰满人妻啪啪|